بررسی تأثیر گونه‌های مختلف باکتری سودوموناس بر عملکرد، اجزای عملکرد و جذب فسفر سه رقم برنج

نوع مقاله : مقاله پژوهشی

نویسندگان

1 عضو هیأت علمی مرکز تحقیقات کشاورزی و منابع طبیعی مازندران، ساری

2 عضو هیأت علمی موسسه تحقیقات خاک و آب، کرج

3 عضو هیأت علمی مؤسسه تحقیقات خاک و آب، کرج

چکیده

استفاده از باکتری­های افزاینده رشد از طریق مکانیسم­های مستقیم و غیر مستقیم می­تواند باعث افزایش رشد و عملکرد در گیاهان زراعی شود. در این آزمایش تأثیر گونه­هایی از باکتری سودوموناس با توان حل کنندگی فسفات بر عملکرد، پارامترهای رشد و جذب فسفر در سه رقم برنج مورد مطالعه قرار گرفت. اثر این گونه­ها از طریق انجام یک آزمایش گلدانی به شکل فاکتوریل در قالب طرح کاملاً تصادفی و در چهار تکرار بررسی شد. در این آزمایش ارقام برنج شامل: طارم، ندا و خزر و شش سویه از باکتری سودوموناس: GO11, GO12, GO15, GU10, MZ3, MZ16 همراه با یک تیمار بدون تلقیح، مورد ارزیابی قرار گرفت. در مرحله گلدهی وزن­تر و خشک اندام هوایی گیاه و جذب فسفر در گیاه، و در مرحله برداشت محصول، عملکرد دانه، وزن هزار دانه، تعداد خوشه، تعداد سنبلچه، تعداد دانه در خوشه، ارتفاع بوته و جذب فسفر در دانه اندازه­گیری شد. نتایج حاصل از اجرای این تحقیق نشان داد که بر اساس آزمون دانکن در سطح پنج درصد، در هر سه رقم مورد مطالعه، اختلاف معنی­داری بین پارامترهای اندازه­گیری شده وجود داشته و بیشترین عملکرد دانه از رقم ندا حاصل شد. همچنین تلقیح با سویه­هایی از باکتری سودوموناس، همه پارامترهای رشد را افزایش داد و سویهPseudomonas fluorescens GO15 ، بیشترین تأثیر را بر عملکرد دانه برنج داشت که نسبت به تیمار بدون تلقیح (شاهد)، 5/24% افزایش نشان داد. نتایج حاصل از اثر متقابل ارقام مختلف برنج و سویه­های باکتری نشان داد که بیشترین عملکرد دانه و پارامترهای رشد از تلقیح سویه GO15 Pseudomonas fluorescens و رقم ندا حاصل شد. جذب فسفر در گیاه و دانه با تلقیح سویهPseudomonas fluorescens GO12 و به ترتیب با رقم ندا و خزر حاصل شد. بر اساس نتایج حاصل از این آزمایش، سویه­های باکتری سودوموناس بر عملکرد دانه برنج و جذب فسفر در گیاه و دانه مؤثر بود.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of Different Strains of Pseudomonads on Yield, Yield Components, and Phosphorus Uptake of Three Rice Cultivars

نویسندگان [English]

  • Mahmoud Reza Ramezanpour 1
  • H. Asadi Rahmani 2
  • K. Khavazi 3
1 Agricultural and Natural Resources Research Center of Mazandaran, IranSoil and Water Department, , Agricultural Natural Resources Research Centre of Mazandran ,Sari, Iran
2 Soil and Water Research Institute, Karaj, Iran
3 Soil and Water Research Institute, Karaj, Iran
چکیده [English]

Plant growth promoting rhizobacteria can enhance growth and yield of different crops through direct and indirect mechanisms. In this experiment, effect of phosphate solubilizing pseudomonads strains on yield, growth parameters and phosphorus uptake of three rice cultivars was studied. The experiment was carried out in a randomized complete design (RCD) in factorial experiment with four replications. Three cultivars of rice Tarom, Neda and Khazar, and six strains of Fluorescent pseudomonads GO11, GO12, GO15, GU10, MZ3 and MZ16 were used along with a control treatment. The collected data included weight of fresh shoot, weight of dry shoot and phosphorus uptake by plant at flowering stage, and yield of grain, 1000 seed weight, number of panicles, number of spikelets, number of grains per panicle, and phosphorus uptake in grain at maturity. Results of the experiment showed that all the parameters according to Duncan test (P ≤ 0.05) were significantly affected in different cultivars. Maximum grain yield was obtained from Neda cultivar. Inoculation with strains increased all plant biometrical parameters, growth indices and yield of rice. Maximum grain yield (30.61g per pot) was obtained with P. fluorescens GO15 strain and showed a significant increase in comparison with the control, (24.5%.). Maximum grain yield and growth indices were obtained from inoculation of seeds of Neda cultivar with P. fluorescens GO15. Maximum rates of P uptake in plant and grain of rice were obtained from interaction of P. fluorescens GO12 with Neda and Khazar cultivars, respectively. Based on the obtained results, fluorescent pseudomonads could affect yield of rice and P uptake in plant and grain.

کلیدواژه‌ها [English]

  • Phosphate solubilizing
  • Growth promoting bacteria
  • Fluorescent pseudomonads
  • Rice grain yield

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پژوهش های خاک
دوره 26، شماره 3
آذر 1391
صفحه 277-288

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