Effect of Plant Growth Promoting Rhizobacteria on Improvement of Nutrition and Growth of Pistachio Seedling under Drought Stress

Document Type : Research Paper

Authors

1 Assistant professor of Shahid Bahonar University of Kerman, College of Agriculture. Soil Science Department

2 Professor. College of Agricultural and Natural Resources, Tehran University

3 Research professor of Agricultural Research Organization, Tehran, Iran

4 Associate professor of University college of Agricultural and Natural resources, Tehran University

Abstract

This research was carried out to evaluate the possibility of using native plant growth-promoting Rhizobacteria (PGPR) for improvement of nutrition and growth of pistachio seedling under drought stress condition. Hence, a greenhouse experiment was performed as factorial arrangement on the basis of completely randomized design (CRD) including seven bacterial treatments and three drought stress levels with four replications. Bacterial treatments included control (T1), inoculation with two IAA producing isolates (T2), one ACC-deaminase producing isolate (T3), two isolates with high phosphate solubilizing potential (T4), four isolates with high siderophore producing potential (T5), mixture of Acc-deaminase and IAA producing isolates (T6) and mixture of all isolates (T7). Drought stress levels included D0, D1, and D2 by using 80%, 50%, and 20 % of available water, respectively. The mean of shoot and root dry matter, total leaf area and number, plant height and root volume significantly decreased by drought stress. Considering the results of means comparison, the maximum shoot weight, plant height, total leaf area and number was obtained in plant inoculated with ACC deaminase producing bacteria (T3), while the minimum was observed in plants with no inoculation (T1). The mixture of ACC deaminase and IAA producing bacteria (T6) had statically similar effects to ACC deaminase producing bacteria in severe drought stress. T3 treatment had the most significant effect on all root parameters under severe drought level. Drought stress resulted in significant increase in concentration and significant decrease in uptake of P, Fe, and Zn by plant. The highest concentration and phosphorus uptake by plants were, respectively, related to T6 and T3 and the minimum belonged to the control (T1). These results suggested that plant inoculation with IAA (T2) and/or ACC deaminase producing bacteria and the mixture of these bacteria could improve certain growth indices in drought stress conditions.

Keywords

References

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Iranian Journal of Soil Research
Volume 27, Issue 1
April 2013
Pages 107-119

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