Release of Potassium and Iron from Biotite and Phosphorus from Tri-calcium Phosphate by Seven Strains of Bacteria under In -Vitro Conditions

Document Type : Research Paper

Authors

1 Former MSc student, Faculty of Agriculture, University of Tabriz

2 Professor, Department of Soil Science, Faculty of Agriculture, University of Tabriz

3 Assistant Professor, Department of Soil Science, Faculty of Agriculture, University of Tabriz

4 Agricultural Sciences and Natural Resources, Sari

Abstract

Phosphorus, potassium, and iron are among the essential nutrients that play an important role in plant growth and development. This study was conducted to investigate the proficiency of 7 different strains of bacteria in solubilizing K, Fe, and P from insoluble minerals in laboratory.  An experiment based on completely randomized design with four replications was conducted under in-vitro conditions. Treatments consisted of seven bacterial strains and control (non-bacterial). Five strains (JK3, JK4, JK5, JK6 and JK7) were the Bacillus megaterium, while strains JK1 and JK2 were the Lysinibacillus fusiformis and  Arthrobacter sp, respectively. Aleksandrov's medium (MA) supplemented with biotite and Pikovskaya medium (PVK) containing tri-calcium phosphate at concentration of 0.2%  and 0.5%, respectively, were used to investigate solubilizing ability of the studied strains. Release of K, Fe, P and the final pH were investigated in their culture media. The inverse relationships between the final pH and the amount of soluble P in their Pikovskaya medium was observed, while, after 5 days incubation, there were no significant differences in the decrease in the pH of the liquid medium in biotite treatment. Solubilizing ability of the bacteria was higher in the case of phosphorus than potassium and iron. Compared with the non-bacterial treatment, all strains solubilized significantly (P< 0.01) higher K and Fe from Aleksandrov's medium; however, the highest P was observed in Pikovskaya medium. Strain JK7 revealed maximum value of solubilized potassium (3.1 mg g-1), but had no significant difference with isolates JK3 and JK6. Also, among the inoculated bacteria, strain JK7 recorded the maximum release of iron from biotite mica (0.29 mg g-1) in Aleksandrov's medium and showed a significant difference with other isolates. Strain JK7 recorded maximum release of phosphorus from Pikovskaya medium (61.26 mg g-1) and showed a significant difference with other strains. Therefore, it can be concluded that the strain JK7 is the most efficient P-, Fe-, and K-solubilizing bacteria under in-vitro condition.

Keywords

References

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Iranian Journal of Soil Research
Volume 27, Issue 4
March 2013
Pages 555-564

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