Effectiveness of IAA and ACC-deaminase Producing Fluorescent Pseudomonads on Growth of Pistachio Seedlings

Document Type : Research Paper

Authors

1 M.Sc., of Soil Science, Vali-e-Asr University of Rafsanjan

2 Assistant Professor, Departmentof Soil Science, Vali-e-Asr University of Rafsanjan

3 Associate Professor Department of Soil Science, Vali-e-Asr University of Rafsanjan

Abstract

The aim of this study was to investigate the effectiveness of isolates capable of producing IAA and utilizing ACC as the sole source of nitrogen on growth of pistachio seedlings. In the present study, based on fluorescent properties of isolates in King B medium, 52 colonies of fluorescent pseudomonad were randomly isolated from the rhizosphereof pistachio seedlings and then purified. These strains were identified according to the microscopic, physiological, and biochemical characteristicstestsrelated to species determination. Study results showed that 49 of the 52 isolates belonged toPseudomonasfluorescens species and, due to the lack of conformitywith the characteristicsin the Bergey book, three isolates wereidentified as Pseudomonas sp. Then, isolates were evaluated for the ability to produce IAA and utilize ACC. The results indicated that all the strainswere able toproduce IAA and 62% ofwhichwereable toutilizeACCas the sole sources of nitrogen. In a greenhouse experiment, the effectiveness of the six isolatesthat were able to utilize ACC and produce different amounts of IAA were tested on the pistachio seedlings. The results showed that application of most of the strains significantly increased leaf number, leaf area, and shoot fresh and dry weight of pistachio seedlings.

Keywords

References

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Iranian Journal of Soil Research
Volume 26, Issue 1
June 2012
Pages 89-97

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