Effect of Fluorescent Pseudomonads on Improving Zinc Compounds Solubility and Zinc Uptake by Common Bean (Phaseolus vulgaris L.)

Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Soil Science, College of Agriculture and Natural Sciences, University of Tehran

2 Associate Professor, Department of Soil Science, College of Agriculture and Natural Sciences, University of Tehran

3 Assistant Professor, Soil and Water Research Institute, Karaj

4 Assistant Professor, Department of Soil Science, College of Agriculture and Natural Sciences, University of Tehran

5 MSc. Plant Pathology

Abstract

Pseudomonads fluorescent is one of the most important plant growth promoting bacteria that increase plant yield and growth through several different mechanisms. They have been reported many times for their ability to dissolve the zinc insoluble compounds. In this research, 40 isolates were evaluated for their ability in solubilization of ZnO, ZnS, and ZnCO3 compounds. The results indicated that 20 percent of the examined isolates could dissolve ZnO and ZnCO3 salts, but none of them could dissolve ZnS. Zinc solubilizing isolates were investigated for their growth stimulating factors.  The results demonstrated that all of the zinc solubilizing isolates could produce auxin and siderophore and they also could dissolve phosphate insoluble compounds in both solid and liquid media. ACC-deaminase was not produced in all of the isolates, except P1. None of the strains could produce HCN except for GRP3. The effect of the selected strains on zinc uptake was determined in greenhouse condition. P19 was selected as the most effective strain. It could increase zinc concentration up to 50.1 mg.kg-1 in shoot, at 500 mg.kg-1 concentration of ZnCO3 . 

Keywords

References

  1. Abbas-Zadeh, P., H. Asadi-Rhmani, N. Saleh-Rastin, K. Khvazi, and A.A. Soltani. 2009. Evaluation of auxin production from fluorescent pseudomonads and their effects on canola (Brassica napus). Iran. J. Soil Water Sci. 22(2): 203-215
  2. Abbas-Zadeh, P., N. Saleh-Rastin, H. Asadi-Rahmani, K. Khavazi, A. Soltani, R. Shoary-Nejati, and M. Miransari. 2010. Plant growth-promoting activities of fluorescent pseudomonads, isolated from the Iranian soils. Acta. Physiol. Plant 32:281–288
  3. Ahmad, F., I. Ahmad, and M. Sahir khan. 2005. Indoleacetic acid production by indigenous isolates of azotobacter and fluorescent pseudomonads in the presence and absence of tryptophan. Turk. J. Biol. 29: 29-34.
  4. Alexander, D.B., and D. A. Zuberer. 1991. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biol. Fertil. Soils. 12: 39-45.
  5. Ali, B., A.N. Sabri, K. Ljung, and S. Hasnain. 2009a. Quantification of indole-3-acetic acid from plant associated Bacillusand their phytostimulatory effect on Vigna radiata (L.).World J. Microbiol. Biotechnol. 25:519–526
  6. Amico, E.D., L. Cavalca, and V. Andreoni. 2005. Analysis of rhizobacterial communities in perennial Graminaceae from polluted water meadow soil, and screening of metal-resistant, potentially plant growth-promoting bacteria. FEMS Microbiol. Ecol. 52: 153-162.
  7. Asghar, H.N., Z.A. Zaeir., and M. Arshad. 2004. Screening rhizobacteria for improving the growth ,yield,and oil cotent of canola (Brassica napus).Aust. J.Agric. Res.55:187-194.
  8. Blumer, C., and D. Hass. 2000. Mechanism, regulation, and ecological role of bacterial cyanide biosenthesis. Arch. Microbiol. 173(3): 170-177.
  9. Cakmak, 2008. Enrichment of cereal grains with zinc: agronomic or genetic biofortification. Plant Soil 302:1–17.
  10. Chen, Y., E. Jurkevitch, E. Bar-Ness, and Y. Hadar. 1994. Stability constants of pseudobactin complexes with transition metals. Soil Sci. Soc. Am. J. 58:390-396.
  11. Cheng, Z., E. Park, and B.R. Glick. 2007. 1-Aminocyclopropane-1-carboxylate (ACC) deaminase from Pseudomonas putida UW4 facilitates the growth of canola in the presence of salt. J. Microbiol. 53(7):912-8.
  12. DiSimine, C.D., J.A. Sayer, and G.M. Gadd. 1998. Solubilization of zinc phosphate by a strain of Pseudomonas fluorescens isolated form a forest soil. Biol. Fertil. Soils 28: 87-94.
  13. Dobbelaere, S., J. Vanderleyden, and Y. Okon. 2003. Plant growth-promoting effects of diazotrophs in the rhizosphere. Critical Reviews in Plant Sciences. 22(2): 107-149.
  14. Donate-Correa, J., M. Leon-Barrios, and R. Perez-Galdona. 2004. Screening for plant growth-promoting rhizobacteria in Chamaecytisus proliferus (tagasaste), a forage tree-shrub legume endemic to the Canary Island. Plant Soil. 266: 261-272.
  15. Echeverria, S.R., M.A.P. Fernandez, S. Vlaar, and T. Finnan. 2003. Analysis of the legume–rhizobia symbiosis in shrubs from central western Spain. J. Appl. Microbiol. 95: 1367–1374.
  16. Glick, B.R. 1995. The enhacement of plant growth by free-living bacteria. J. Microbiol. 41: 109-117.
  17. Hotz, C., and KH. Brown. 2004. Assessment of the risk of zinc deficiency in populations and options for its control. Food Nutr. Bull. 25:94–204.
  18. Huang, X.D. 2004. A multi-process phytoremediation system for removal of polycyclic aromatic hydrocarbons from contaminated soils.Environ. Pollut. 130: 465–476.
  19. Iqbal, U., N. Jamil, Ali, and H. Hasnain. 2010. Effect of zinc-phosphate-solubilizing bacterial isolates on growth of Vigna radiata. Ann. Microbiol. 60:243–248.
  20. Jeon, J.S., S.S. Lee, H.Y. Kim, T.S. Ahn, and H.G. Song. 2003. Plant growth promoting in soil by some inoculated microorganism. J. Microbiol.271-276.
  21. Kalbasi, M., G.J. Racz, and A. Lewen-Rudgers. 1978. Reaction products and solubility of applied zinc compounds in some Manitoba soils. Soil Sci. 125:55–64.
  22. Meyer, J.M. 2000. Pyoverdins: pigments, siderophores and potential taxonomic markers of fluorescent Pseudomonas Arch. Microbiol. 174: 135-142
  23. O,Sullivan, D.J., and F. O,Gara. 1992. Traits of Pseudomonas fluorescens Involved in suppression of plant root pathogens. Microbial. Rev. 56: 662-676.
  24. Ping, L., and W. Boland. 2004.Signals from the underground: bacterial volatiles promote growth in Arabidopsis.Trends. plant. Sci.9: 263-266.
  25. Raj, A. 2002. Biofertilizers for Micronutrients. Biofert. News Lett. 10:8–10.
  26. Rashid, M.S., N. Khalil, S. Ayub, S. Alam, and F. Latif. 2004. Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions.Pak.J. Biol. Sci.7(2) 187-196.
  27. Saharan, B.S,. and V. Nehra. 2011. plant Growth Promoting Rhizobacteria: A Critical Review. Life Sci. Med. Res. 21:1-30
  28. Saleem, M., M. Arshad, S. Hussain, and A. Bhatti. S.2007. Perspective of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase in stress agriculture. J. Ind. Microbiol. Biotechnol. 34:635–648.
  29. Sarathambalm C., M. Thangaraju, C. Paulraj, and M. Gomathy. 2010. Assessing the Zinc solubilization ability of Gluconacetobacter diazotrophicus in maize rhizosphere using labelled 65Zn compounds. Indian J. Microbiol.50 (1):103-109.
  30. Saravanan, V.S., S.R. Subramoniam, and A. Ra. 2003. Assessing in vitro solubilization potential of different zinc solubilizing bacterial (zsb) isolates. Braz. J. Microbiol. 34:121-125.
  31. Seshadre, S., Muthukumarasamy, R., Lakshminarasimhan, C. And Ignaacimuthu, S. 2002. Solubilization of inmorganic phosphates by azospirillum halopraeferans. Curr. Sci. 79(5): 565-567.
  32. Shahab, S., and N. Ahmed. 2008. Effect of various parameters on the efficiency of zinc phosphate solubilization by indigenous bacterial isolates. Afr. J. Biotechnol. 7(10): 1543-1549.
  33. Singh, M.V. 2001. Evaluation of current micronutrient stocks in different agro ecological zones of India for sustainable crop production. Fert. News 46:25–28.
  34. Sundara, B., V. Natarajan, and K. Hari. 2002. Influence of phosphorus solubilizing bacteria on the changes in soil available phosphorus and sugarcane and sugar yields. Field Crop Res.77:43-49.
  35. Tolay, I., B. Erenoglu, and Cakmak. 2001. Phytosiderophore release in Aegilopsis and Triticum species under zinc and iron deficiencies. J. Exper. Bot. 52:1093-1099.
  36. Zaidi, S., S. Usmani, B.R. Singh, and J. Musarrat. 2006. Significance of Bacillus subtilis strain SJ-101 as a bioinoculant for concurrent plant growth promotion and nickel accumulation in Brassica juncea. Chemosphere 64: 991–997.
  37. Zhang, S., M.S. Reddy, and J.W. Kloepper. 2002. Development of Assays for Assessing Induced Systemic Resistance by Plant Growth-Promoting Rhizobacteria against Blue Mold of Tobacco. Biol. Control 23: 79–86.

 

Iranian Journal of Soil Research
Volume 26, Issue 2
September 2012
Pages 195-206

Files

Share

How to cite

Statistics