Nutrient Uptake and Yield of Canola (Brassica napus L.) in Saline Condition as Affected by Fluorescent Pseudomonads with ACC Deaminase Activity

Document Type : Research Paper

Authors

1 Assistant Professor, Islamic Azad University,Khoy branch

2 Associate Professor, Soil and Water Research Institute

3 Professor, Islamic Azad University,Science and Research branch

Abstract

Salt accumulation in arid and semiarid areas of the world is one of the most common abiotic stresses that can have a wide range of effects on crop yield. The objective of this research was evaluation of the effect of fluorescent pseudomonads containing ACC-deaminase on nutrient uptake and growth of canola (Brassica napus L.) in saline condition. The experiment was conducted in factorial arrangement with completely randomized design with four replications. The experimental variables were: water salinity at four EC levels of 0.335 (control), 5, 10, and 15 dS/m (natural saline water) and five levels of bacterial inoculants including Pseudomonas putida strain P.p. 169, P.p.196 and Pseudomonas fluorescens strain P.f. 108, P.f. 11  and  a non-inoculated control. The results showed that inoculation with the selected strain was effective at alleviating salinity stress during different growth stages of canola. By increasing the salinity, uptake of K and Ca decreased, while uptake of Na and Cl increased. At salinity levels, inoculation with the selective strain, decreased the effects of salt stress, hence, inoculation of canola seeds with fluorescent pseudomonads strain may alleviate the stress effects of salinity on canola growth and nutrient uptake. 

Keywords


  1. بی نام.1381. دستورالعمل آبشویی خاک­های شور و سدیمی ایران، انتشارات کمیته ملی آبیاری و زهکشی، نشریه فنی شماره 255.
  2. امامی،ع. 1375. روش­های تجزیه گیاه، تحقیقات خاک و آب، نشریه فنی شماره 79.
  3. جلیلی،ف.،ک.خاوازی، ه.اسدی رحمانی.1390. تأثیر سودوموناس­های فلورسنت با فعالیت آنزیم ACC- دآمیناز بر شاخص­های رشد کلزا در شرایط شور، مجله دانش خاک و آب،ج.21. ش.2. صفحات188-175.
  4. علی احیایی،م.1378. روش های تجزیه خاک،موسسه تحقیقات خاک و آب، نشریه فنی شماره 273.
  5. ذبیحی،ح.، غ.ر.ثوابقی،ک. خاوازی، ع.گنجعلی.1388.تأثیر سودوموناس­های فلورسنت بر عملکرد و اجزاع عملکرد گندم در سطوح مختلف شوری، مجله خاک و آب،ج.23.ش.1. صفحات 208-199.
  6. Bacilio, M., H. Rodriguez, M. Moreno, J. P. Hernandez, and Y. Bashan. 2004. Mitigation of salt stress in wheat seedling by agfp-taged Azospirillum lipoferum. Biol. Fertil. Soils, 40:188-193.
  7. Cheng, Z.,E. Park, and B.R.Glick. 2007. 1- Aminocyclopropane-1-carboxylate deaminase from Pseudomonase putida UW4 faciliate the growth of canola in the precence of salt. Can. J. Microbiol., 53: 912-918.
  8. D. 2007. The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Appl. Soil. Ecol., 36:184-189.
  9. Farwell, A. J., S. Vesely, V. Nero, H. Rodriguez, K. McCormack, S. Shah, D. G. Dixon, and B. R. G. Glick. 2007. Tolerance of transgenic canola plants (Brassica napus L.) amended with plant growth-promoting bacteria to flooding stress at a metal-contaminated field site, Environ. Pollut. 147 : 540e545.
  10. Gamalero, E., G. Lingua, G. Berta, and B. G. Glick. 2009. Beneficial role of plant growth promoting bacteria and arbuscular mycorrhizal fungi on plant responses to heavy metal stress. Can. J. Microbiol., 55: 501-514.
  11. Glick, B. R. 1995. The enhancement of plant growth promotion by free living bacteria.Can. J. Micribiol., 41:109-117.
  12. Glick, B. R., D. Karaturovic, and P. Newell. 1995. A novel procedure for rapid isolation of plant growth-promoting rhizobacteria. Can. J. Microbiol. 41: 533–536.
  13. Glick, B. R., D. M. Penrose, and J. Li. 1998. A model for the lowering of plant ethylene concentration by plant growth promoting bacteria. J. Theor. Biol., 190:63-68.
  14. Gosh, S., J.N. Penterman, R.D. Little, R. Chavez, and B.R.Glick. 2003. Three newly isolated plant growth-promoting bacilli facilitate the seedling growth of canola
  15. (Brassica campestris). Plant Physiology and Biotechnology, 41: 277-281.
  16. V.P., B. Filby, and B. R. Glick. 2000. Increased of transgenic plants expressing the bacterial enzyme ACC deaminase to accumulate Cd, Co, Cu, Ni, Pb, and Zn. J. Biotech., 81:45-53.
  17. Grichko, V. P., and B. R. Glick. 2001. Amelioration of flooding stress by ACC deaminase-containing plant growth promoting bacteria. Plant Physiol. Biochem., 39:11-17.
  18. Hall,J.A., D.Peirson, S.Ghosh, andB.R.Glick. 1996. Root elongation in various agronomic crops by the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 . Israel J. Plant Sci. 44: 37- 42.
  19. Hamaoui, B., J. M. Abbadi, S. Burdman, A. Rashid, S. Sarig, and Y. Okon. 2001. Effects of inoculation with Azospirillum brasilense on chickpeas(Cicer arietinum) and faba beans(Vicia faba) under different growth condition. Agronomie, 21:553-560.
  20. Hamdi, M. A., M. A. K. Shaddad, and M. M. Doaa. 2004. Mechanisms of salt tolerance and interactive effects of Azospirillum brasilense inoculation on maize cultivars grown under salt stress conditions. Plant Growth Regulation, 44: 165–174.
  21. Hamdia , M.A., and El-Komy , H.M. 1997. Effects of salinity, gibberelic acid and Azosbirillum inoculation on growth and nitrogen uptake of Zea mays. Plant 40:109-120
  22. Han, H. S., and K. D. Lee. 2005a. Plant Growth Promoting Rhizobacteria Effect on Antioxidant Status, Photosynthesis,Mineral Uptake and Growth of Lettuce under Soil Salinity. Res. J. Agric. Biol. Sci., 1: 210-215.
  23. Han, H.S., and K.D. Lee. 2005b. Physiological Responses of Soybean - Inoculation of Bradyrhizobium japonicum with PGPR in Saline Soil Conditions. Res. J. Agric. Biol. Sci., 1: 216-221.
  24. Husnain, S., and A. N. Sabri. 1996. Growth stimutation  of Triticum  aestivum  seedling under  Cr – stress  by nonrhizospheric Pseudomonas strain . In: Abstract Book of 7th int Symp . on Nitrogen fixation with  Non – legumes . Faisalabad , Pakistan , pp . 36.
  25. Jalili,F., K. Khavazi, E. Pazira, H. Asadi Rhmani, A. R. Najati, H. Rasouli, and M. Miransari. 2008. Isolation and characterization of ACC deaminase producing fluorescent pseudomonads, to alleviate salinity stress on canola (Brassica napus L.) growth, J. Plant Physiol., 166:667-674.
  26. Klopper, J. W. 2003. A review of mechanisms for plant growth promotion by PGPR. 6th international PGPR workshop 5-10 october 2003 calculla, India.
  27. Kuffner, M., M. Puschenreiter, G. Wieshammer, M. Gorfer, and A. Sessitsch. 2008. Rhizosphere bacteria affect growth and metal uptake of heavy metal accumulating willows, Plant Soil, 304:35-44.
  28. Munns, R. 2002. Comparative physiology of salt and water stress. Plant Cell Environ., 25:239–250.
  29. Mayak, S., T. Tirosh, and B. G. Glick .2004a. Plant Growth promoting bacteria confer resistance in tomato plants salt stress .Plant Physiol. Biochem. 42:565-572.
  30. Mayak, S., T. Tirosh, and B. G. Glick. 2004b. Plant growth promoting bacteria that confer resistance to water stress in tomatoes and peppers. Plant Sci., 166:524-530.
  31. Nadeem,S., Z.A. Zahir,M.Naveed and M.Arshad. 2007.Preliminary inverstigations on inducing salt tolerance in Canola through ACC-deaminase activity.Canadian Journal of Microbiology.53(10)1141-1149.
  32. Nadeem, S. M., Z. A. Zahir, M. Naveed, M. Arshad, and S.M. Shahzad. 2006. Variation in growth and ion uptake of maize due to inoculation with plant growth promoting rhizobacteria under salt stress, Soil , 25: 78-84.
  33. Nandakumar, R., S. Babu, R.Viswanathan, T. Raguchander, and R. Samiyappan. 2001. Induction of systemic resistance in rice against sheath blight disease by plant growth promoting rhizobacteria. Soil Biol. Biochem., 33: 603–612.
  34. Netondo, G.W., J.C. Onyango , and E. Beck. 2004. Sorghum and salinity.I: Response of growth, water relations, and ion accumulation to NaCl salinity. Crop Sci., 44:797–805.
  35. Neel, J. P. S., G. Alloush, A. D. P. Belesky, and W. M. Clapham. 2002. Influence of rhizosphere ionic strength on mineral composition, dry matter yield and nutritive value of forage chicory. J. Agron. Crop Sci., 188: 398–407.
  36. Poustini, K., and Siosemardeh. 2004. Ion distribution in wheat cultivars in response to salinity stress. Field Crops Res., 85:125-133.
  37. Rasouli Sadagiani, M.H., K.Khavazi, H. Rahimiyan, and M.J.Malakuti.2005. Evaluation of siderophor production activity by pseudomonas fluorescens in wheat rhizospher, J. Soil Water(in Persian),20(1):135-143.
  38. ReyhaniTabar,A., N. Saleh Rastin. H.A.Alikhani, and M.Mohammadi. 2002. The effects of pseudomonas fluorescens on nutrients uptake in wheat, J. Agric. Sci.(in Persian),33(4):771-780.
  39. Shaharoona, B., M. Arshad, and Z. A. Zahir. 2006. Effect of plant growth promoting rhizobacteria containing ACC deaminase on maize (Zea mays ) growth under axenic conditions and on nodulation in mung bean (Vigna radiata L.). Lett.Appl. Microbiol., 42: 155-159.
  40. Vivas, A., A. Marulanda, J.M. Ruiz-Lozano, J. M. Barea, and R. Azcon. Influence of a Bacillus sp. on physilogical activities of two arbuscular mycorrhizal fungi and on plant response to PEG-induced drought stress. Mycorriza, 13: 249-256.
  41. Waqar, A., B. Shahroona, Z. A. Zahir, and M. Arshad. 2004. Inoculation with ACC deaminase Containing Rhizobacteria for improving growth and yield of wheat. Pak. J. Agri. Sci., 41:119-124.