Use of ACC Deaminase –Containing Fluorescent Pseudomonads to Alleviate Effects of Salinity on Canola (Brassica napus L.) Growth in Germination Stage

Document Type : Research Paper

Authors

1 Ph.D Student of Soil Science, Science and Research Branch of Tehran Azad University

2 Assistant Professor, Soil and Water Research Institute( SWRI)

3 Professor of Science and Research Branch of Tehran Azad University

4 Microbiologist, Soil and Water Research Institute

Abstract

Salinity stress in some arid and semi-arid areas is of great importance due to its potential negative impact on crop yield. Under salinity stress the amount of 1-aminocyclopropane-1-carboxylate (ACC), a precursor for ethylene in plant, increases. Hence, a research work was planned hypothesizing that ACC deaminase producing Pseudomonas fluorescens and putida may alleviate  the  harmful  effects  of  salinity  on  canola  (Brassica  napus  L.) growth. The experiments were conducted in the Soil and Water Research Institute,  Tehran,  Iran.  Seven  stages  of  experiments  were  conducted  to isolate and characterize ACC Deaminase producing P. fluorescens species and to determine factors, enhancing their growth and, hence, their effects on the germination of canola seeds. Under salinity stress, in 14% of the isolates, ACC deaminase activity was observed, indicating that they are able of utilizing ACC as the sole source of N. Bacterial species differed in their ability to synthesize auxin like compounds, hydrogen cyanide and ACC deaminase. Under salinity stress the rate of germinated seeds, inoculated with the species of ACC Deaminase producing P. fluorescens and putida, was significantly higher. Hence, inoculation of canola seeds with P.fluorescens and putida may alleviate the stress of salinity on canola seed germination and, perhaps, canola growth.

Keywords

References

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Iranian Journal of Soil Research
Volume 23, Issue 1
March 2009
Pages 91-105

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