Evaluation of Auxin Production From Fluorescent Pseudomonads and Their Effects on Growth of Canola (Brassica napus L.)

Document Type : Research Paper

Authors

1 Graduate Student, Tehran University

2 Assistant Professor, Soil and Water Research Institute

3 Associate Professor, Tehran University

4 Former Graduate Student, Tehran University

Abstract

For assessment of auxin production from fluorescent Pseudomonads and effects of their inoculation on growth of canola seedlings, a study was conducted using 40 strains of Pseudomonads. Fifteen strains had been originally isolated from rhizosphere of wheat as well as two other strains GRP3 and MPFM were obtained from microbial culture collection of Soil and Water Research Institute (SWRI). Twenty – three strains were isolated from rhizosphere soil samples of canola fields from Karaj region. Bacterial strains were isolated by soil serial dilation culture technique on king B agar plates and were identified using morphological and biochemical tests. Two media, enriched TBS and DF minimal salt supplemented with 0, 50, 100 and 200 mg.l-1 of L-TRP were employed to evaluate the efficiency of strains for production of auxin. The strains were further tested for their effects on canola growth in a sand-based pot experiment. Results revealed that auxin biosynthesis was enhanced as L-TRP concentration was increased in culture medium. Strain P23 was the most effective in auxin production in DF medium supplemented with 200 mg.l-1 of L-TRP. Bacterial strains significantly increased growth of canola.The highest correlation was observed between auxin production in DF minimal salt medium with 50 mg.l-1 of L-TRP and shoot dry weight of plants. These findings imply that the inoculation with auxin producing fluorescent Pseudomonads could promote growth of canola. 

Keywords

References

  1. رسولی صدقیانی، ح.، ک. خاوازی.، ح. رحیمیان.، م. ج. ملکوتی و ه. اسدی رحمانی. 1384. بررسی تراکم جمعیت و شناسایی سودوموناس­های فلورسنت در ریزوسفر گندم مناطق مختلف ایران. مجله خاک و آب. جلد 19، شماره 2، مؤسسه تحقیقات خاک و آب، تهران، ایران
  2. Ahmad, F., I. Ahmad, and M. Sahir khan. Indoleacetic acid production by indigenous isolates of azotobacter and fluorescent pseudomonas in the presence and absence of tryptophan. Turkish Journal of Biology, 29: 29-34
  3. Asghar, H.N. Z.A., Zaeir, and M. Arshad. 2004. Screening rhizobacteria for improving the growth, yield and oil cotent of canola (Brassica napus). Australian Journal of Agriculture Research, 55:187-194.
  4. Asghar, H.N., Z.A. Zahir, M. Arshad and A. Khaliq. 2002. Relationship between in vitro production of auxins by rhizobacteria and their groth-promoting activities in Brassica juncea Biology and Fertility of Soils, 35:231-237.
  5. Bashan, Y. and H. Levanony. 1990. Current status of Azospirillum inoculation technology: Azospirillium as a challenge for agriculture. Canadian Journal of Microbiology, 36: 591-608.
  6. Benizri, E., A. Courtade, C. Picard and A. Guckert. 1998. Role of maize root exudates in the production of auxin by Pseudomonas flurescense3.1. Soil Biology and Biochemistry, 30(10/11): 1481-1484.
  7. Bent, E., S. Tuzan, C.P. Chanway and S. Enebak. 2001. Alteration in plant growth and in root hormone levels of lodgepole pines inoculated with rhizobacteria. Canadian Journal of Microbidogy, 47:793-800.
  8. Bossis, E., P. Lemanceau, X. Latour and L. Gradan. 2000. The taxonomy of pseudomonas fluorescens and pseudomonas putida :Current status and need for revision. Agronomic, 20:51-63.
  9. Dobbelaere, S., A. Croonenborghs, A. Thys, A.V. Broek and J. Vanderleyden. 1999. Phytostimulatory effect of Azospirillum brasilense wild type and mutant strains altered in IAA production on wheat. Plant and Soil, 212: 155-164.
  10. Dobbelaere, S., J. Vanderleyden and Y. Okon. 2003. Plant growth-promoting effects of diazotrophs in the rhizosphere. Critical Reviews in Plant Sciences, 22(2):
    107-149.
  11. Elmerich, C. 1984. Molecular biology and ecology of diazotrophs associated with non-leguminous plants. Biotechnology, 2: 967-978.
  12. Frankenberger, W.T. and W. Brunner. 1983. Method of detection of auxin- 3-acetic acid in soil by high performance liquid chromatography. Soil Science Society of American Journal, 47: 237-241.
  13. Fuentes-Ramirez, L.E., T. Jimenez-Salgado, L.R. Abarca-Qcampo and L.Caballero- Mellado. 1993. Acetobacter diazotrophicus, an indole acetic production bacterium isolated from sugarcane cultivars of mexico. Plant and Soil, 154: 145-150.
  14. Glick, B. R. 1995. The enhacement of plant growth by free-living bacteria. Canadian Jourmal of Microbiology, 41: 109-117.
  15. Glick, B. R., C. B. Jacobson, M. M. K. Schwarze and J. J. Pasternak. 1994. 1-Aminocyclopropane-1-Carboxylix acid deaminase mutants of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 do not stimulate canola root elongation. Canaolian Journal of Microbioligy, 40: 911-915.
  16. Jean, J.S., S.S. Lee, H.Y. Kim, T.S. Ahn and H.G. Song. 2003. Plant growth promoting in soil by some inoculated microorganism. Journal of Microbiology, 41(4): 271-276.
  17. Kloepper, J. W., R. Lifshitz and M. N. Schroth. 1988. Pseudomonas inoculants to benefit plant production. ISI. Atlas of Science: Animal and Plant Science, 1:60-64.
  18. Kloepper, J. W., R. R. Lifshitz and R. M. Zablotwicz. 1989. Free-living bacterial inocula for enhancing crop productivity. Trends Biotechnology. 7: 39-43.
  19. Kloepper, J.M. 2003. A review of mechanisms for plant growth promoting by pgpr. Aurburn University, Auburn, Alabama 36849, USA.
  20. Lambrecht, M., Y. Okon, A.V. Broek and J. Vanderleyden. 2000. Indole-3- acetic acid: a reciprocal signaling molecule in bacteria-plant interaction. Trends Microbiology, 8(7):298-300.
  21. Omay, S.H., W.A. Schmidt, P. Martin and F. Bangerth. 1993. Indole acetic acid production by the rhizosphere bacterium Azospirillum brasilinse Cd under in vitro conditions. Canadian Journal of Microbiology, 39: 187-192.
  22. Patten, C.L., B. R. Glick. 1996. Bacterial biosynthesis of indole-3-acetic acid. Canadian Journal of Microbiology, 42: 207-220.
  23. Patten, C.L., B.R. Glick. 2002. Role of pseudomonas putida indoleacetic acid in development of host plant root system. Applied and Environmental Microbiology, 68: 3795-3801.
  24. Penrose, M., R. Glick. 2003. Methods for isolating and characterizing Acc deaminase-containing plant growth-promoting rhizobacteria. Plant Physiology, 118: 10-15.
  25. Raju, R. A. and M. N. Reddy. 1999. Effect of rock phosphate amended with phosphate solubilizing bacteria and farmyard manure in wetland (Oryza sativa). Indian Journal of Agricultural Science, 69: 451-453.
  26. Rubio, T.M.G., S.A. Valencia-Plata, J. Bernal-Castillo and P. Martinez-Nieto. 2000. lsolation of Enterobacteria, Azotobacter And Pseudomonas sp. producers of indole-3-acetic acid and siderophores, from colombian rice rhizosphere. Revista Latinoamericana de Microbiologia, 42: 171-176.
  27. Sarwar, M., and W.T. Frankenberger. 1994. Influence of L-trypyophan and auxins applied to the rhizosphere on the vegetative growth of Zea mays Plant and Soil, 160: 97-104.
  28. Sarwar, M. and R.J. Kremer. 1995. Enhanced suppression of plant growth through production of L.tryptophan- derived compounds by deleterious rhizobacteria. Plant and Soil, 172: 261-269.
  29. Sarwer, M., M. Arshad, A.M. Martens and W.T. Frankenberger. 1992. Trytophan-dependent biosynthesis of auxins in soil. Plant and Soil, 147: 207-215.
  30. Tang, W. H. 1994. Yield-increasing bacteria and biocontrol of sheath blight of rice. Organisation, Adelaide, Australia, 267-278.
  31. Tolay, I., B. Erenoglu and Cakmak. 2001. Phytosiderophore release in Aegilopsis and Triticum species under zinc and iron deficiencies. Journal of Experimental Botany, 52:1093-1099.
  32. Vlassak, K., L. Vanholm, L. Duchateau, J. Vanderleyden and R. Demot. 1992. Isolation and characterization of fluorescent pseudomonads associated with the roots of rice and banana grown in srilanka. Plant and Soil, 145:51-63.
Iranian Journal of Soil Research
Volume 22, Issue 2
February 2009
Pages 203-215

Files

Share

How to cite

Statistics