Isolation and Determination of PGP Characteristics in Some of Indigenous Fluorescent Pseudomonads of Iranian Soils

Document Type : Research Paper

Authors

1 Former Graduate Student, Soil Science Department, Tehran University

2 Associate professor, Tehran University;

3 Assistant Professor, Soil and Water Research Institute

Abstract

In this research, twenty-five isolates of fluorescent pseudomonads were isolated from forty soil samples of wheat rhizosphere and their ability for promoting plant growth were determined. Results of the study revealed that all the isolates were able to produce auxin (ranging from 1.3-4.5 g/ml) with an average of 2.44. The range of inorganic P solubilization was 129.9-386.1 g/ml and the average was 287.5. Nine isolates were capable of utilizing ACC as carbon and or nitrogen sources, whereas all of them produced siderophore on both CAS agar and CASAD plates. The range of siderophore production was 0.072-0.459 mM of deferoxamine mesylate salt with an average of 0.21 none of the isolates were capable of chitinase production. Salicylic acid production by the isolates ranged from 0-10.91 g/ml (average of 2.48). Bacterial ability for production of hydrogen cyanide had a great variation and based on this ability they were qualitatively classified in to very high, high, intermediate and low. Results of this study showed that fluorescent pseudomonads are native to soils of Iran have the potential to be used as plant growth promoters. There is also a need to study their effects on different agricultural crops. 

Keywords

References

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Iranian Journal of Soil Research
Volume 21, Issue 2
February 2008
Pages 277-289

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