Application of Agrobactrium rhizogenes to Form Inductive Roots in Some Dicotyledonous Plants

Document Type : Research Paper

Authors

1 post-graduate of azzahra universsity

2 masterof azzahra university

3 Scintific staff of soil and water research institue

4 Expert of soil and water research institue

Abstract

In this investigation 3 strains of Agrobactrium rhizogenes (A4S, A4V and a strain assigned to this species) were used to inoculate 7 types of plant tissues including carrot disks, leaflets and cotyledons of bean, the stem of bean seedling, internodal section of tissue- culture propagated potato stems, radicle of alfalfa and internodal areas of the stems of alfalfa plant. In carrot disks, leaflets and stem of bean and potato clusters of hairy roots were observed at the site of inoculation. Calculation of the percentage of appearace of hairy roots in different plants by different strains of A.rhizogenes indicated an interaction between strains of the bactrium and the species of the plants used. 

Keywords


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