Effect of Inoculation with Arbuscular Mycorrhizal Fungi on Biomass and Uptake of Nutrients of Hazelnut Seedlings in Field Condition

Document Type : Research Paper

Authors

1 Forests and Rangelands Research Department, Ardabil Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Ardabil, Iran

2 Assoc., Prof., Forest Research Department, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran

Abstract

Mycorrhizal fungi are one of the useful soil microorganisms, which can increase the growth of the host plant by absorbing and transferring more water and nutrients to the plant due to symbiosis with plant roots. Therefore, in order to investigate the effect of mycorrhizal fungi on the biomass and nutrient absorption of hazelnut seedlings, a factorial experiment was conducted in the form of a randomized complete block design in field conditions. The investigated factors included the three origins of the seedlings (Fandoghlou, Makesh, and Makidi regions) and fungal inoculation in three levels (Rhizophagus irregularis, Myco root inoculum and control) with three replications of 25 at a distance of 3x3 m, in an area of 6075 m2 in the agricultural lands on the edge of Fandoghlou Forest in Ardabil, Iran. After four years, the results showed that the inoculated seedlings of all three origins were superior in terms of all the examined characteristics compared to the control (non-inoculated) seedlings. The highest traits belonged to Fandoghlou seedlings with R. irregularis inoculation. In these seedlings, root colonization, dry mass of root, stem and leaf were, respectively, 51.9%, 54.4%, 45.1%, and 40.7% higher, and N, P, K, Fe, and Zn were, respectively, 38.8%, 45.4%, 62.9%, 35.5%, and 42.6% more compared to the control seedlings. Finally, it can be concluded that planting of hazelnut seedlings inoculated with mycorrhizal fungi (especially R. irregularis) will have higher vegetative growth.

Keywords

Main Subjects

References

  1. Augé, R.M. 2004. Arbuscular mycorrhizae and soil/plant water relations. Can. J. Soil Sci. 84:373–381.
  2. Baraloto, C., Forget, P.M., and D.E. Goldberg. 2005. Seed mass, seedling size and neotropical tree seedling establishment. J. Ecol. 53: 1-10.
  3. Barna, T. 2003. The role of mycorrhizae in afforestation. VIIth International symposium interdisciplinary regional research – ISIRR 2003. Hungary – Serbia & Montenegro- Romania, 25th – 26th, September,
  4. Begum, N., Qin, C., Ahanger, M.A., Raza, S., Khan, M.I., Ashraf, M., Ahmed, N. and L. Zhang.  2019. Role of Arbuscular Mycorrhizal Fungi in Plant Growth Regulation: Implications in Abiotic Stress Tolerance. Front. Plant Sci. 10:1068.
  5. Bi, Y., Xie, L., Wang, Z., Wang, K. and W. Liu. 2021. Arbuscular mycorrhizal symbiosis facilitates apricot seedling (Prunus sibirica) growth and photosynthesis in northwest China. Int. J. Coal Sci. Technol. 8: 473-482.
  6. Caravaca, F., Barea, J.M., Figueroa, D. and A. Roldán. 2002a. Assessing the effectiveness of mycorrhizal inoculation and soil compost addition for enhancing reafforestation with Olea europaea sylvestris through changes in soil biological and physical parameters. Appl. Soil Ecol. 20: 107-118.
  7. Caravaca, F., Barea, J.M. and A. Roldán. 2002b. Synergistic influence of an arbuscular mycorrhizal fungus and organic amendment on Pistacia lentiscus seedlings afforested in a degraded semiarid soil. Soil Biol. Biochem. 34 (8): 1139-1145.
  8. Caravaca, F., Alguacil. M.M., Barea, J.M. and A. Roldán. 2005. Survival of inocula and native AM fungi species associated with shrubs in a degraded Mediterranean ecosystem. Soil Biol. Biochem. 37(2):227-233
  9. Cornell, C., Kokkoris, V., Turcu, B., Dettman, J., Stefani, F. and N. Corradi. 2022. The arbuscular mycorrhizal fungus Rhizophagus irregularis harmonizes nuclear dynamics in the presence of distinct abiotic factors. Fungal Genet Biol.158:103639.
  10. Delgado, T., Malheiro, R., Pereira, J.A. and E. Ramalhosa. 2010. Hazelnut (Corylus avellana) kernels as a source of antioxidants and their potential in relation to other nuts. Ind. Crops Prod. 32: 621–626.
  11. Estaun, V., Camprub, A. and C. Calvet. 2003. Nursery and field response of olive tree inoculated with two arbuscular mycorrhiza fungi Glomus intraradices and Glomus mosseae. J. Am. Soc. Hortic. Sci. 128 (5): 767-775.
  12. Giovannetti, M. and B. Mosse. 1980. An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol. 84: 489-500.
  13. Hilszczanska, D., Sierota, Z. and M. Palenzona. 2008. New Tuber species found in Poland. Mycorrhiza .18: 223–226.
  14. Kandeler, E., Marschner, P., Tscherko, D., Gahoonia, T.S., Nielsen, N.E. 2002. Microbial community composition and functional diversity in the rhizosphere of maize. Plant and Soil. 238: 301–312.
  15. Issac, R.A. and W.C. Johnson. 1975. Colloborative study of wet and dry techniques for the elemental analysis of plant tissue by atomic absorption spectrometer. J. Assoc. Agri. Chem. 58: 336-340.
  16. Jackson, M.L. 1967. Soil Chemical Analysis. Prentice Hall Inc., Englewood cliffs, NJ, USA, 521 p
  17. Pizo, M.A., Allmen, C.V., Patricia, L., and C. Morellato .2006. Seed size variation in the palm Euterpe edulis and the effects of seed predators on germination and seedling survival. Acta Oecol. 29: 1- 4.
  18. Phillips, J. M. and D. S. Hayman. 1970. Improved procedure for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. TBMS. 55: 158-161.
  19. Razouk, R., and A. Kajji. 2015. Effect of Arbuscular Mycorrhizal Fungi on Water Relations and Growth of Young Plum Trees under Severe Water Stress Conditions. Int. J. Plant Sci. 5(5): 300-312.
  20. Seiwa, K. 2000. Effects of seed size and emergence time on tree seedling establishment: importance of developmental constraints. Oecologia. 123: 1-7.
  21. Smith, S.E. and D.J. Read. 2008. Mycorrhizal Symbiosis. Academic Press, London
  22. Tadesse, C. S. and A. T. Fassil. 2013. Arbuscular mycorrhizal fungi associated with shade trees and Coffea Arabica in a coffee-based agroforestry system in Bonga, Southwestern Ethiopia. Afrika Focus. 26 (2): 111-131.
  23. Sylvia, D.M. 1986. Spatial and temporal distribution of vesicular-arbuscular mycorrhizal fungi associated with Uniola paniculata in Florida foredunes. Mycologia, 78(5): 728-734.
  24. Turjaman, M., Santoso, E., Sitepu, I. R., Tawaraya, K., Purnomo, E., Tambunan, R. and M. Osaki. 2009. Mycorrhizal fungi increased early growth of tropical tree seedlings in adverse soil. Indones. J. For. Res. 6 (1): 17-25.
  25. Wahab, A., Muhammad, M., Munir, A., Abdi, G., Zaman, W., Ayaz, A., Khizar, C. and S.P.P. Reddy. 2023. Role of Arbuscular Mycorrhizal Fungi in Regulating Growth, Enhancing Productivity, and Potentially Influencing Ecosystems under Abiotic and Biotic Stresses. Plants. 12, 3102.
  26. Wang, W., Shi, J., Xie, Q., Jiang, Y., Yu, N. and E. Wang. 2017. Nutrient Exchange and Regulation in Arbuscular Mycorrhizal Symbiosis. Mol. Plant. 10: 1147–1158.
  27. Wang, M., Wang, Z., Guo, M., Qu, L. and A. Biere. 2023. Effects of arbuscular mycorrhizal fungi on plant growth and herbivore infestation depend on availability of soil water and nutrients. Front. Plant Sci. 14:1101932.
  28. Williams, A., Norton, D.A. and H.J. Ridgway. 2012. Different arbuscular mycorrhizal inoculants affect the growth and survival of Podocarpus cunninghamii restoration plantings in the Mackenzie Basin, New Zealand. 50 (4): 473-479.
  29. Zhang, H., Liu, Z., Chen, H. and M. Tang. 2016. Symbiosis of arbuscular mycorrhizal fungi and Robinia pseudoacacia L. improves root tensile strength and soil aggregate stability. PLoS ONE 11: e0153378.
  30. Zhang, H., Wei, S., Hu, W., Xiao, L. and M. Tang. 2017. Arbuscular Mycorrhizal Fungus Rhizophagus irregularis Increased Potassium Content and Expression of Genes Encoding Potassium Channels in Lycium barbarum. Front. Plant Sci. 8:440.
Iranian Journal of Soil Research
Volume 37, Issue 3
December 2023
Pages 277-290

Files

Share

How to cite

Statistics