پالایش میکروبی خاک‌های آلوده به مواد نفتی و بررسی نقش رایزوسفر در کارایی ریز جانداران

Oil contamination is one of the most common and usual kinds of pollution in aquatic and terrestrial ecosystems. One of the economical and effective methods for decreasing or eliminating oil contamination is using plants and microorganisms. Soils contain a wide variety of oil decomposing microorganisms, of which pseudomonad, Rhodocoous and Nocardia are the most important. To evaluate the effect of oil decomposing bacteria on bioremediation of oil contaminated soils with and without plant (the role of rhizosphere), this research was conducted in greenhouse condition. Fifteen soil samples form depth of 0-30 cm were collected from around an oil refinery, where soils were exposed to oil contamination for a long time. Oil decomposing bacteria were isolated and purified from the collected soil samples, then their bioremediation efficiency was determined. A greenhouse experiment was conducted as completely randomized design using bacteria (4 levels), plant (2 levels) and pollutant (4 levels) as variable factors. Sixty days after beginning of the pot experiment, wheat plants were cut and fresh and dry weight of their root and shoot and also the concentration of pollutants in soil were determined. The results indicated significant effects of bacteria, pollution, and their interactive effect on shoot fresh weight, as well as the effects of pollution and interaction of bacteria with pollution on shoot dry weight, with similar effects of pollution on fresh and dry weight of root. Pollution decreased the fresh and dry weight of root and shoot, while inoculation of bacteria improved root and shoot weight, due to elimination of pollutant. The effects of all treatments on pollutant concentration were significant. Planting and bacteria inoculation decreased pollutant concentration in soil significantly. Bacteria were more efficient than the plant in decreasing soil pollutant, and plant did not increase bacterial efficiency significantly. Thus, the difference between plant and plant+bacteria treatments was not significant.