تأثیر سودوموناس‌های فلورسنت بر افزایش انحلال ترکیبات روی و بهبود جذب آن توسط لوبیا (Phaseolus vulgaris L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه مهندسی علوم خاک، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

2 دانشیار گروه مهندسی علوم خاک، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

3 استادیار پژوهش موسسه تحقیقات خاک و آب

4 استادیار گروه مهندسی علوم خاک، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

5 فارغ‌التحصیل کارشناسی ارشد بیماری‌شناسی گیاهی از دانشگاه تهران

چکیده

باکتری­های سودوموناس فلورسنت از مهمترین باکتری­های ریزوسفری محرک رشد گیاه می­باشند که از طریق سازو­کارهای متعدد می­توانند سبب افزایش رشد و عملکرد گیاهان شوند. در این تحقیق 40 سویه از سودوموناس­های فلورسنت از نظر توان انحلال نمک­های مختلف روی شامل ZnO، ZnS و ZnCO3 مورد ارزیابی قرار گرفتند. سویه­های حل­کننده روی از نظر صفات محرک رشدی نیز مورد ارزیابی قرار گرفتند. نتایج نشان داد که 20 درصد سویه­های مورد آزمایش توان انحلال نمک­های ZnCO3 و ZnO را دارا بودند و هیچ یک از سویه­های باکتریایی نتوانستند ZnS را به ­صورت محلول درآورند. تمامی سویه­های حل­کننده روی توانایی تولید اکسین، سیدروفور و انحلال ترکیبات نامحلول فسفر را در محیط جامد و مایع دارا بودند. در بین سویه­های مورد آزمایش تنها سویه 1P توان تولید آنزیم ACC-دآمیناز داشت. آزمایش تولید سیانید هیدروژن نشان داد که در بین 8 سویه تنها GRP3 توانایی تولید سیانید هیدروژن را دارد. تأثیر سویه­های منتخب در بهبود جذب روی در یک آزمون گلخانه­ای مورد بررسی قرار گرفت. بر اساس نتایج حاصله، سویه­ی 19P به عنوان کارآترین سویه توانست در غلظت 500 میلی­گرم در گیلوکرم نمک ZnCO3، غلظت روی را تا 1/50 میلی­گرم در کیلوگرم اندام هوایی افزایش دهد. با توجه به آهکی بودن اکثر خاک­ها در ایران، بخش عمده کودهای محلول روی به صورت غیرقابل استفاده در می­آید. استفاده از این باکتری­ها می­تواند در افزایش کارایی کودهای شیمیایی روی در خاک موثر باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Fluorescent Pseudomonads on Improving Zinc Compounds Solubility and Zinc Uptake by Common Bean (Phaseolus vulgaris L.)

نویسندگان [English]

  • P. Abbaszedeh 1
  • G.R. . Savaghebi 2
  • H. Asadi Rahmani 3
  • F. Rejali 3
  • Mohsen Farahbakhsh 4
  • Babak Motesharezadeh 4
  • M. Omidvari 5
1 Ph.D. Student, Department of Soil Science, College of Agriculture and Natural Sciences, University of Tehran
2 Associate Professor, Department of Soil Science, College of Agriculture and Natural Sciences, University of Tehran
3 Assistant Professor, Soil and Water Research Institute, Karaj
4 Assistant Professor, Department of Soil Science, College of Agriculture and Natural Sciences, University of Tehran
5 MSc. Plant Pathology
چکیده [English]

Pseudomonads fluorescent is one of the most important plant growth promoting bacteria that increase plant yield and growth through several different mechanisms. They have been reported many times for their ability to dissolve the zinc insoluble compounds. In this research, 40 isolates were evaluated for their ability in solubilization of ZnO, ZnS, and ZnCO3 compounds. The results indicated that 20 percent of the examined isolates could dissolve ZnO and ZnCO3 salts, but none of them could dissolve ZnS. Zinc solubilizing isolates were investigated for their growth stimulating factors.  The results demonstrated that all of the zinc solubilizing isolates could produce auxin and siderophore and they also could dissolve phosphate insoluble compounds in both solid and liquid media. ACC-deaminase was not produced in all of the isolates, except P1. None of the strains could produce HCN except for GRP3. The effect of the selected strains on zinc uptake was determined in greenhouse condition. P19 was selected as the most effective strain. It could increase zinc concentration up to 50.1 mg.kg-1 in shoot, at 500 mg.kg-1 concentration of ZnCO3 . 

کلیدواژه‌ها [English]

  • Zinc solubilizing bacteria
  • Auxin
  • Siderophore

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پژوهش های خاک
دوره 26، شماره 2
شهریور 1391
صفحه 195-206

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